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KMID : 0043319950180060391
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Archives of Pharmacal Research
1995 Volume.18 No. 6 p.391 ~ p.395
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Kainate-induced Elevations of Intracellular Ca++ and Extracellular Glutamate are Partially Decreased by NMDA Receptor Antagonists in Cultured Cerebellar Granule Neurons
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Oh Sei-Kwan
Shogo-Tokuyama
Patrick P.McCaslin
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Abstract
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Several lines of evidence indicate that physiological activity of N-methyl-D-aspartate (NMDA) receptor was blocked by physiological concentration of (1.2 mM). However, the activity of NMDA receptor may not be blocked totally with this concentration of under elevated membrane potential by kainate. Here, we described the effect of on NMDA receptor and how much of NMDA receptor functions could be activated by kainate. Effects of NMDA receptor antagonist on kainate-induced elevation of intracellualr levels and extracellular glutamate level were examined in cultured rat cerebellar granule neurons. kainate-induced elevation of was not affected by physiological concentration of . Kainate-induced NMDA-induced elevation was blocked by the same concentration of Kainate-induced elevation of [ was decreased by 32% in the presence of NMDA antagonists, MK-801 and CPP (3-[2-carboxypiperazine-4-yl]propyl-1-phosphonic acid), in free buffer. Kainate receptor-activated gluamate release was also decreased (30%) by MK-801 or CPP. These resuts show that certain extent of elevations of intracellular and extracellular glutamate by kainate is due to coativation of NMDA receptors.
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KEYWORD
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N-methyl-D-aspartate, Kinate, Ca++ influx, Glutamate release, Cerebellar granule neuron
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